Abstract

Background: Rice black-streaked dwarf virus (RBSDV) and Southern rice black-streaked dwarf virus (SRBSDV) are two closely related fijiviruses transmitted by the small brown planthopper (SBPH) and white-backed planthopper (WBPH), respectively. SRBSDV has a latent period 4 days shorter than that of RBSDV, implying a more efficient spread in insect vector. Currently, the mechanisms underlying this higher efficiency are poorly understood. However, our recent studies have implicated a role of virus induced tubular structures in the dissemination of fijiiruses within their insect vectors.

Methods: Immunofluorescence labeling was performed to visualize and compare the dynamics of P7-1 tubule formation of the RBSDV and SRBSDV in their own vector insects and nonhost Spodoptera frugiperda (Sf9) cells.

Results: Tubule formation of SRBSDV P7-1 was faster than that of RBSDV P7-1. For RBSDV, P7-1 formed tubules were observed at 3-days post-first access to diseased plants (padp) in SBPH. For SRBSDV, these structures were detected as early as 1 day padp in WBPH. Importantly, similar phenomena were observed when P7-1 proteins from the two viruses were expressed alone in Sf9 cells.

Conclusions: Our research revealed a relationship between the speed of P7-1 tubule formation and virus dissemination efficiency and also supports a role of such tubular structures in the spread of reoviruses within insect vectors. Keywords: Rice black-streaked dwarf virus, Southern rice black-streaked dwarf virus, Tubule formation, Dissemination efficiency, Latent period.

论文链接：

http://download.springer.com/static/pdf/88/art%253A10.1186%252Fs12985-016-0632-1.pdf?originUrl=http%3A%2F%2Fvirologyj.biomedcentral.com%2Farticle%2F10.1186%2Fs12985-016-0632-1&token2=exp=1492504830~acl=%2Fstatic%2Fpdf%2F88%2Fart%25253A10.1186%25252Fs12985-016-0632-1.pdf*~hmac=05132869e9ae38c60b5546d2202b1ce45fb8e53d924b5b31a20da77f31d28344